Anti-Ran Monoclonal Antibody

Product Name

Anti-Ran Monoclonal Antibody

View all RAN Antibodies

SKU/Catalog Number

M00204

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Ran Monoclonal Antibody catalog # M00204. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ran Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00204)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACFI-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Ran GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity) .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00204 is reactive to RAN in Human, Mouse, Rat

Applications

M00204 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

103 kDa

Calculated molecular weight

24.423kDa

Background of RAN

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:100

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

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Western blot analysis of Ran expression in (1)HeLa cell lysate; (2)RAW264.7 cell lysate.

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Immunofluorescent analysis using the Antibody at 1:500 dilution.

Gene/Protein Information For RAN (Source: Uniprot.org, NCBI)

Alternative Names

Androgen receptor-associated protein 24; ARA24Gsp1; GTPase Ran; GTP-binding nuclear protein Ran; guanosine triphosphatase Ran; member RAS oncogene family; OK/SW-cl.81; RAN, member RAS oncogene family; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein; TC4 RAN ARA24, Gsp1, TC4 RAN, member RAS oncogene family GTP-binding nuclear protein Ran|GTPase Ran|RanGTPase|androgen receptor-associated protein 24|guanosine triphosphatase Ran|member RAS oncogene family|ras-like protein TC4|ras-related nuclear protein

For more info on RAN, check out the RAN Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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